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American Society of Microbiology ABMM Sample Questions (Q160-Q165):

NEW QUESTION # 160
A research laboratory is investigating the quorum sensing system in Pseudomonas aeruginosa. They identify a novel autoinducer molecule and a corresponding transcriptional regulator. Disruption of the gene encoding this regulator leads to a significant decrease in the production of virulence factors such as elastase and pyocyanin. Further analysis reveals that this regulator directly binds to the promoter regions of these virulence genes in the presence of the autoinducer. This regulatory mechanism is an example of:

A. A two-component regulatory system involving a sensor kinase and a response regulator.
B. A global regulatory network controlled by alternative sigma factors.
C. A direct transcriptional activation mechanism dependent on a small signaling molecule.
D. A post-transcriptional regulatory mechanism involving small regulatory RNAs.

Answer: C

NEW QUESTION # 161
A clinical microbiology laboratory is implementing a new multiplex nucleic acid amplification test (NAAT) for the detection of respiratory viral pathogens. To ensure the quantitative accuracy of the assay, it is essential to include:

A. Internal controls to monitor for PCR inhibition.
B. Calibration standards with known concentrations of target nucleic acids.
C. External quality assessment samples.
D. Only positive and negative controls.

Answer: B

NEW QUESTION # 162
Vancomycin resistance in Enterococcus faecium, mediated by the vanA gene cluster, results from:

A. Modification of the drug target from D-alanyl-D-alanine to D-alanyl-D-lactate
B. Decreased outer membrane permeability to vancomycin
C. Efflux pump overexpression removing vancomycin from the cell
D. Enzymatic inactivation of vancomycin by phosphorylation

Answer: A

NEW QUESTION # 163
A microbiology laboratory is performing antimicrobial susceptibility testing using Etest strips. The MIC value is determined by:

A. The lowest concentration at which the elliptical zone of inhibition intersects the strip.
B. The presence or absence of growth at a single critical concentration.
C. The diameter of the zone of inhibition around the strip.
D. The highest concentration that allows visible bacterial growth within the zone of inhibition.

Answer: A

NEW QUESTION # 164
A clinical microbiology laboratory is using isothermal nucleic acid amplification techniques for rapid detection of Mycobacterium tuberculosis in sputum. Compared to conventional PCR, isothermal methods offer the advantage of:

A. Easier integration with downstream analysis techniques such as Sanger sequencing.
B. Faster reaction times and the ability to be performed without sophisticated thermocyclers.
C. Higher sensitivity and specificity for detecting low levels of target DNA.
D. The ability to simultaneously amplify multiple targets in a single reaction.

Answer: B

NEW QUESTION # 165
......

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